NSCI 601 - Spring 2008  -  Topics in Synaptic Transmission                 close window  

Tuesdays 1:00 - 3:50  -  NE403 Med/Dent 
Barry Wolfe  bwolfe01@georgetown.edu 


Assignments of specific papers to specific students will not be done.  
Everyone will be expected to participate on each paper.

Be sure you understand
a.  The overall goals of the paper
b.  The specific conclusions of the paper
You should annotate each figure in your copy of the paper to remind yourself in class regarding:
a.  The purpose of the experiment for each figure
          (why did they do this expt?)
b.  The methods used in each figure
          (what did they do in this expt?)
c.  The conclusions reached from the results of each figure
          (what did they learn from this expt?)

If you have problems understanding something, please come talk to me in advance
___________________________________________________________

January 22  -   Neurexin/Neuroligin interactions

Paper to Read:

1.  Neurexin mediates the assembly of presynaptic terminals.  Nature Neuroscience 6: 708, 2003

Background Papers to skim:

R1.   Neuroligin 1 is a postsynaptic cell-adhesion molecule of excitatory synapses.  Proc. Natl. Acad. Sci. 96: 1100, 1999

R2.      Neuroligin expressed in nonneuronal cells triggers presynaptic development in contacting neurons.  Cell 101: 657, 2000

Good Review

Li and Sheng:  Some Assembly Required.  Nature Reviews Mol. Cell. Biol. 4: 833, 2003

 January 29  -  Making synapses in non-neuronal cells

Paper to Read: 

1.    Functional excitatory synapses in HEK293 cells expressing neuroligin and glutamate receptors.  J. Neurophysiol. 90: 3950, 2003

Background Paper to skim:

Febrary 5  -  It's not just glutamate!

Paper to Read: 

1.    Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins.  Cell 119: 1013-1026, 2004


February 19 -  Rebekah
Main papers -  Chubykin et al.  Activity Dependent Validation of Excitatory versus Inhibitory Synapses by Neuroligin-1 
                                         versus Neuroligin-2.   
                                         Neuron 54:919, 2007
           This paper proposes a new hypothesis for what the neuroligin-neurexin interaction is doing with 
            regard to synapse formation.  It rejects the current hypothesis that they participate in the initial
            formation of the synapse.
Reviews and background - Chih et al.  Alternative splicing controls selective trans-synaptic interactions of the 
                                                                          neuroligin-neurexin complex.
                                                                          Neuron 51:171, 2006
                                                This paper presents data that says certain splice variants of neuroligin-1 and -2 can 
                                                 promote the 'other' type of synapse.  Thus splicing controls whether a synapse becomes
                                                 glutamatergic or GABAergic.    Rebekah's paper disagrees  AND calls into question the 
                                                 validity of using the HEK cell 'synapse'.
February 26 -   Rebekah
Main papers -  Shankar GM, Bloodgood et al.  Natural oligomers of the Alzheimer amyloid-beta protein induce reversible 
                                         synapse loss by modulating an NMDA-type glutamate receptor-dependent signaling pathway. 
                                         J Neurosci. 27:2866, 2007 
Reviews and background - 

March 4 - Spring Break
March 11 - Rebekah
Main paper - Dunah AW &  Standaert DG. Dopamine D1 receptor-dependent trafficking of striatal NMDA glutamate receptors
                                     to the postsynaptic membrane. 
                                     J Neurosci.  21:5546,  2001
Reviews and background - 

March 18 - Scott
Main paper - Marchoionni et al.  In the developing rat hippocampus a tonic GABAA-mediated conductance selectively 
                                     enhances the glutamatergic drive of principal cells.  
                                     J. Physiol.  581:515, 2007
Reviews and background - 
March 25 - Scott
Main paper - Lemtiri-Chlieh and Levine.  Lack of depolarization-induced suppression of inhibition (DSI) in layer 2/3 
                                     interneurons that receive cannabinoid-sensitive inhibitory inputs
                                     J. Neurophysiol. 98:2517, 2007
Reviews and background - 
April 1 - Scott 
Main paper - Stevens et al.   The classical complement cascade mediates CNS synapse elimination
                                     Cell 131:1034, 2007
Reviews and background - Huh et al.  Functional requirement for class I MHC in CNS development and plasticity
                                                                          Science 290:2051, 2000

April 8 - Patrick
Main paper - Lindemann et al. Trace Amine-Associate Receptor 1 Modulates Dopaminergic Activity.  
                           J. Pharmacol. Exp. Ther. 324:948, 2008
April 15 - Patrick
Main paper - Shindou et al.  Actions of adenosine A2A receptors on synaptic 
                          connections of spiny projections neurons in the neostriatal inhibitory network.
                           J. Neurophysiol. 2008  Feb 13 Epub ahead of print
April 22  -   Patrick

Main paper -  Zhang et al.  In vivo regulation of Homer1a expression in the striatum by cocaine.  
                            Mol Pharmacol 71:1148, 2007
 
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